Differential RNA editing landscapes in host cell versus the SARS-CoV-2 genome

Differential RNA editing landscapes in host cell versus the SARS-CoV-2 genome

Blog Article

Summary: The SARS-CoV-2 pandemic was defined by the emergence of new variants formed through virus mutation originating from random errors not corrected by viral proofreading and/or the host antiviral response introducing mutations into the viral genome.While sequencing information hints at Milk Thistle (Silymarin) cellular RNA editing pathways playing a role in viral evolution, here, we use an in vitro human cell infection model to assess RNA mutation types in two SARS-CoV-2 strains representing the original and the alpha variants.The variants showed both different Grubs and Worms cellular responses and mutation patterns with alpha showing higher mutation frequency with most substitutions observed being C-U, indicating an important role for apolipoprotein B mRNA editing catalytic polypeptide-like editing.

Knockdown of select APOBEC3s through RNAi increased virus production in the original virus, but not in alpha.Overall, these data suggest a deaminase-independent anti-viral function of APOBECs in SARS-CoV-2 while the C-U editing itself might function to enhance genetic diversity enabling evolutionary adaptation.